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non-specific control sirna  (Thermo Fisher)


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    Structured Review

    Thermo Fisher non-specific control sirna
    Non Specific Control Sirna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/non-specific+control+sirna/nonspecific+control+sirna/pm40334825-79-8-14
    Average 90 stars, based on 1 article reviews
    non-specific control sirna - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Control:

    Article Title: Effect of isolated keratin 3 knockdown on gene expression of primary limbal epithelial cells without and with inflammatory stimuli.
    Article Snippet: Control LECs were transfected with 150 pmol of non-specific control siRNA (Catalogue no. #4390843, Thermo Fisher Scientific, SilencerTM Select Negative Control, Waltham, USA).

    Article Title: CCN2 Binds to Tubular Epithelial Cells in the Kidney
    Article Snippet: HK2 cells were silenced using either a predesigned siRNA targeting the human EGFR or a non-specific control siRNA, both FAM-labeled (Ambion, Fisher Scientific, Waltham, MA, USA).

    Article Title: Calcium Channels as Novel Therapeutic Targets for Ovarian Cancer Stem Cells
    Article Snippet: After seeding the cells, they were transfected with siGENOME SMARTpool siRNA against CACNA1D , CACNA1F , CACNA1H , and non-specific control siRNA (each 100 nM, Thermo Fisher Scientific, USA) using Lipofectamin RNAiMax transfection reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions.

    Article Title: Activation of Protein Tyrosine Phosphatase Non-Receptor Type 2 by Spermidine Exerts Anti-Inflammatory Effects in Human THP-1 Monocytes and in a Mouse Model of Acute Colitis
    Article Snippet: Non-specific control siRNA (100 pmol/transfection, Life Technologies Ltd) was used as negative control.

    Article Title: Functional diversity of miR-146a-5p and TRAF6 in normal and oral cancer cells.
    Article Snippet: A non-specific control siRNA (Invitrogen, Carlsbad, CA, USA) and siRNAs against human TRAF6 (40 nM; Sigma-Aldrich, St. Louis, MO, USA) and FADD (40 nM; Santa Cruz Biotechnology, Santa Cruz, CA, USA) were used for gene silencing.

    Article Title: Gremlin Regulates Tubular Epithelial to Mesenchymal Transition via VEGFR2: Potential Role in Renal Fibrosis
    Article Snippet: Gene silencing in cultured cells was performed using either a predesigned siRNA corresponding to the human KDR/VEGFR2 cDNA sequence (s7822; Ambion) or a non-specific control siRNA (Ambion).

    Article Title: TLR3 and TLR7 RNA Sensor Activation during SARS-CoV-2 Infection
    Article Snippet: Further, siRNAs specific to human TLR3 (assay ID 107054; Thermo Fisher, Milan, Italy) and human TLR7 (assay ID 108895; Thermo Fisher, Milan, Italy), and the non-specific control siRNA (Ambion Silencer Negative Control) (Thermo Fisher, Milan, Italy), were transfected to Calu-3/MRC5 cells cultured on a 6-well plate (6 × 10 5 /well) using the Lipofectamine RNAiMAX reagent (Thermo Fisher, Milan, Italy) according to the manufacturer’s instructions.

    Article Title: Highly expressed lncRNA H19 in endometriosis promotes aerobic glycolysis and histone lactylation
    Article Snippet: Transient H19 knockdown was performed by transfecting 10 nmol/L siRNA targeting human H19 (Life Technologies, Carlsbad, CA, USA) or non-specific control siRNA (Life Technologies) into HESCs for 48 h using Lipofectamine 3000 (L3000001, Invitrogen, USA) according to the manufacturer's instructions.

    Transfection:

    Article Title: Effect of isolated keratin 3 knockdown on gene expression of primary limbal epithelial cells without and with inflammatory stimuli.
    Article Snippet: Control LECs were transfected with 150 pmol of non-specific control siRNA (Catalogue no. #4390843, Thermo Fisher Scientific, SilencerTM Select Negative Control, Waltham, USA).

    Article Title: CCN2 Binds to Tubular Epithelial Cells in the Kidney
    Article Snippet: HK2 cells were silenced using either a predesigned siRNA targeting the human EGFR or a non-specific control siRNA, both FAM-labeled (Ambion, Fisher Scientific, Waltham, MA, USA).

    Article Title: Calcium Channels as Novel Therapeutic Targets for Ovarian Cancer Stem Cells
    Article Snippet: After seeding the cells, they were transfected with siGENOME SMARTpool siRNA against CACNA1D , CACNA1F , CACNA1H , and non-specific control siRNA (each 100 nM, Thermo Fisher Scientific, USA) using Lipofectamin RNAiMax transfection reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions.

    Article Title: Activation of Protein Tyrosine Phosphatase Non-Receptor Type 2 by Spermidine Exerts Anti-Inflammatory Effects in Human THP-1 Monocytes and in a Mouse Model of Acute Colitis
    Article Snippet: Non-specific control siRNA (100 pmol/transfection, Life Technologies Ltd) was used as negative control.

    Article Title: Functional diversity of miR-146a-5p and TRAF6 in normal and oral cancer cells.
    Article Snippet: A non-specific control siRNA (Invitrogen, Carlsbad, CA, USA) and siRNAs against human TRAF6 (40 nM; Sigma-Aldrich, St. Louis, MO, USA) and FADD (40 nM; Santa Cruz Biotechnology, Santa Cruz, CA, USA) were used for gene silencing.

    Article Title: Gremlin Regulates Tubular Epithelial to Mesenchymal Transition via VEGFR2: Potential Role in Renal Fibrosis
    Article Snippet: Gene silencing in cultured cells was performed using either a predesigned siRNA corresponding to the human KDR/VEGFR2 cDNA sequence (s7822; Ambion) or a non-specific control siRNA (Ambion).

    Article Title: TLR3 and TLR7 RNA Sensor Activation during SARS-CoV-2 Infection
    Article Snippet: Further, siRNAs specific to human TLR3 (assay ID 107054; Thermo Fisher, Milan, Italy) and human TLR7 (assay ID 108895; Thermo Fisher, Milan, Italy), and the non-specific control siRNA (Ambion Silencer Negative Control) (Thermo Fisher, Milan, Italy), were transfected to Calu-3/MRC5 cells cultured on a 6-well plate (6 × 10 5 /well) using the Lipofectamine RNAiMAX reagent (Thermo Fisher, Milan, Italy) according to the manufacturer’s instructions.

    Article Title: Highly expressed lncRNA H19 in endometriosis promotes aerobic glycolysis and histone lactylation
    Article Snippet: Transient H19 knockdown was performed by transfecting 10 nmol/L siRNA targeting human H19 (Life Technologies, Carlsbad, CA, USA) or non-specific control siRNA (Life Technologies) into HESCs for 48 h using Lipofectamine 3000 (L3000001, Invitrogen, USA) according to the manufacturer's instructions.

    Negative Control:

    Article Title: Effect of isolated keratin 3 knockdown on gene expression of primary limbal epithelial cells without and with inflammatory stimuli.
    Article Snippet: Control LECs were transfected with 150 pmol of non-specific control siRNA (Catalogue no. #4390843, Thermo Fisher Scientific, SilencerTM Select Negative Control, Waltham, USA).

    Article Title: CCN2 Binds to Tubular Epithelial Cells in the Kidney
    Article Snippet: HK2 cells were silenced using either a predesigned siRNA targeting the human EGFR or a non-specific control siRNA, both FAM-labeled (Ambion, Fisher Scientific, Waltham, MA, USA).

    Article Title: Calcium Channels as Novel Therapeutic Targets for Ovarian Cancer Stem Cells
    Article Snippet: After seeding the cells, they were transfected with siGENOME SMARTpool siRNA against CACNA1D , CACNA1F , CACNA1H , and non-specific control siRNA (each 100 nM, Thermo Fisher Scientific, USA) using Lipofectamin RNAiMax transfection reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions.

    Article Title: Activation of Protein Tyrosine Phosphatase Non-Receptor Type 2 by Spermidine Exerts Anti-Inflammatory Effects in Human THP-1 Monocytes and in a Mouse Model of Acute Colitis
    Article Snippet: Non-specific control siRNA (100 pmol/transfection, Life Technologies Ltd) was used as negative control.

    Article Title: Functional diversity of miR-146a-5p and TRAF6 in normal and oral cancer cells.
    Article Snippet: A non-specific control siRNA (Invitrogen, Carlsbad, CA, USA) and siRNAs against human TRAF6 (40 nM; Sigma-Aldrich, St. Louis, MO, USA) and FADD (40 nM; Santa Cruz Biotechnology, Santa Cruz, CA, USA) were used for gene silencing.

    Article Title: Gremlin Regulates Tubular Epithelial to Mesenchymal Transition via VEGFR2: Potential Role in Renal Fibrosis
    Article Snippet: Gene silencing in cultured cells was performed using either a predesigned siRNA corresponding to the human KDR/VEGFR2 cDNA sequence (s7822; Ambion) or a non-specific control siRNA (Ambion).

    Article Title: TLR3 and TLR7 RNA Sensor Activation during SARS-CoV-2 Infection
    Article Snippet: Further, siRNAs specific to human TLR3 (assay ID 107054; Thermo Fisher, Milan, Italy) and human TLR7 (assay ID 108895; Thermo Fisher, Milan, Italy), and the non-specific control siRNA (Ambion Silencer Negative Control) (Thermo Fisher, Milan, Italy), were transfected to Calu-3/MRC5 cells cultured on a 6-well plate (6 × 10 5 /well) using the Lipofectamine RNAiMAX reagent (Thermo Fisher, Milan, Italy) according to the manufacturer’s instructions.

    Article Title: Highly expressed lncRNA H19 in endometriosis promotes aerobic glycolysis and histone lactylation
    Article Snippet: Transient H19 knockdown was performed by transfecting 10 nmol/L siRNA targeting human H19 (Life Technologies, Carlsbad, CA, USA) or non-specific control siRNA (Life Technologies) into HESCs for 48 h using Lipofectamine 3000 (L3000001, Invitrogen, USA) according to the manufacturer's instructions.

    Knockdown:

    Article Title: Effect of isolated keratin 3 knockdown on gene expression of primary limbal epithelial cells without and with inflammatory stimuli.
    Article Snippet: Control LECs were transfected with 150 pmol of non-specific control siRNA (Catalogue no. #4390843, Thermo Fisher Scientific, SilencerTM Select Negative Control, Waltham, USA).

    Article Title: CCN2 Binds to Tubular Epithelial Cells in the Kidney
    Article Snippet: HK2 cells were silenced using either a predesigned siRNA targeting the human EGFR or a non-specific control siRNA, both FAM-labeled (Ambion, Fisher Scientific, Waltham, MA, USA).

    Article Title: Calcium Channels as Novel Therapeutic Targets for Ovarian Cancer Stem Cells
    Article Snippet: After seeding the cells, they were transfected with siGENOME SMARTpool siRNA against CACNA1D , CACNA1F , CACNA1H , and non-specific control siRNA (each 100 nM, Thermo Fisher Scientific, USA) using Lipofectamin RNAiMax transfection reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions.

    Article Title: Activation of Protein Tyrosine Phosphatase Non-Receptor Type 2 by Spermidine Exerts Anti-Inflammatory Effects in Human THP-1 Monocytes and in a Mouse Model of Acute Colitis
    Article Snippet: Non-specific control siRNA (100 pmol/transfection, Life Technologies Ltd) was used as negative control.

    Article Title: Functional diversity of miR-146a-5p and TRAF6 in normal and oral cancer cells.
    Article Snippet: A non-specific control siRNA (Invitrogen, Carlsbad, CA, USA) and siRNAs against human TRAF6 (40 nM; Sigma-Aldrich, St. Louis, MO, USA) and FADD (40 nM; Santa Cruz Biotechnology, Santa Cruz, CA, USA) were used for gene silencing.

    Article Title: Gremlin Regulates Tubular Epithelial to Mesenchymal Transition via VEGFR2: Potential Role in Renal Fibrosis
    Article Snippet: Gene silencing in cultured cells was performed using either a predesigned siRNA corresponding to the human KDR/VEGFR2 cDNA sequence (s7822; Ambion) or a non-specific control siRNA (Ambion).

    Article Title: TLR3 and TLR7 RNA Sensor Activation during SARS-CoV-2 Infection
    Article Snippet: Further, siRNAs specific to human TLR3 (assay ID 107054; Thermo Fisher, Milan, Italy) and human TLR7 (assay ID 108895; Thermo Fisher, Milan, Italy), and the non-specific control siRNA (Ambion Silencer Negative Control) (Thermo Fisher, Milan, Italy), were transfected to Calu-3/MRC5 cells cultured on a 6-well plate (6 × 10 5 /well) using the Lipofectamine RNAiMAX reagent (Thermo Fisher, Milan, Italy) according to the manufacturer’s instructions.

    Article Title: Highly expressed lncRNA H19 in endometriosis promotes aerobic glycolysis and histone lactylation
    Article Snippet: Transient H19 knockdown was performed by transfecting 10 nmol/L siRNA targeting human H19 (Life Technologies, Carlsbad, CA, USA) or non-specific control siRNA (Life Technologies) into HESCs for 48 h using Lipofectamine 3000 (L3000001, Invitrogen, USA) according to the manufacturer's instructions.

    In Vitro:

    Article Title: Effect of isolated keratin 3 knockdown on gene expression of primary limbal epithelial cells without and with inflammatory stimuli.
    Article Snippet: Control LECs were transfected with 150 pmol of non-specific control siRNA (Catalogue no. #4390843, Thermo Fisher Scientific, SilencerTM Select Negative Control, Waltham, USA).

    Article Title: CCN2 Binds to Tubular Epithelial Cells in the Kidney
    Article Snippet: HK2 cells were silenced using either a predesigned siRNA targeting the human EGFR or a non-specific control siRNA, both FAM-labeled (Ambion, Fisher Scientific, Waltham, MA, USA).

    Article Title: Calcium Channels as Novel Therapeutic Targets for Ovarian Cancer Stem Cells
    Article Snippet: After seeding the cells, they were transfected with siGENOME SMARTpool siRNA against CACNA1D , CACNA1F , CACNA1H , and non-specific control siRNA (each 100 nM, Thermo Fisher Scientific, USA) using Lipofectamin RNAiMax transfection reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions.

    Article Title: Activation of Protein Tyrosine Phosphatase Non-Receptor Type 2 by Spermidine Exerts Anti-Inflammatory Effects in Human THP-1 Monocytes and in a Mouse Model of Acute Colitis
    Article Snippet: Non-specific control siRNA (100 pmol/transfection, Life Technologies Ltd) was used as negative control.

    Article Title: Functional diversity of miR-146a-5p and TRAF6 in normal and oral cancer cells.
    Article Snippet: A non-specific control siRNA (Invitrogen, Carlsbad, CA, USA) and siRNAs against human TRAF6 (40 nM; Sigma-Aldrich, St. Louis, MO, USA) and FADD (40 nM; Santa Cruz Biotechnology, Santa Cruz, CA, USA) were used for gene silencing.

    Article Title: Gremlin Regulates Tubular Epithelial to Mesenchymal Transition via VEGFR2: Potential Role in Renal Fibrosis
    Article Snippet: Gene silencing in cultured cells was performed using either a predesigned siRNA corresponding to the human KDR/VEGFR2 cDNA sequence (s7822; Ambion) or a non-specific control siRNA (Ambion).

    Article Title: TLR3 and TLR7 RNA Sensor Activation during SARS-CoV-2 Infection
    Article Snippet: Further, siRNAs specific to human TLR3 (assay ID 107054; Thermo Fisher, Milan, Italy) and human TLR7 (assay ID 108895; Thermo Fisher, Milan, Italy), and the non-specific control siRNA (Ambion Silencer Negative Control) (Thermo Fisher, Milan, Italy), were transfected to Calu-3/MRC5 cells cultured on a 6-well plate (6 × 10 5 /well) using the Lipofectamine RNAiMAX reagent (Thermo Fisher, Milan, Italy) according to the manufacturer’s instructions.

    Article Title: Highly expressed lncRNA H19 in endometriosis promotes aerobic glycolysis and histone lactylation
    Article Snippet: Transient H19 knockdown was performed by transfecting 10 nmol/L siRNA targeting human H19 (Life Technologies, Carlsbad, CA, USA) or non-specific control siRNA (Life Technologies) into HESCs for 48 h using Lipofectamine 3000 (L3000001, Invitrogen, USA) according to the manufacturer's instructions.

    Binding Assay:

    Article Title: Effect of isolated keratin 3 knockdown on gene expression of primary limbal epithelial cells without and with inflammatory stimuli.
    Article Snippet: Control LECs were transfected with 150 pmol of non-specific control siRNA (Catalogue no. #4390843, Thermo Fisher Scientific, SilencerTM Select Negative Control, Waltham, USA).

    Article Title: CCN2 Binds to Tubular Epithelial Cells in the Kidney
    Article Snippet: HK2 cells were silenced using either a predesigned siRNA targeting the human EGFR or a non-specific control siRNA, both FAM-labeled (Ambion, Fisher Scientific, Waltham, MA, USA).

    Article Title: Calcium Channels as Novel Therapeutic Targets for Ovarian Cancer Stem Cells
    Article Snippet: After seeding the cells, they were transfected with siGENOME SMARTpool siRNA against CACNA1D , CACNA1F , CACNA1H , and non-specific control siRNA (each 100 nM, Thermo Fisher Scientific, USA) using Lipofectamin RNAiMax transfection reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions.

    Article Title: Activation of Protein Tyrosine Phosphatase Non-Receptor Type 2 by Spermidine Exerts Anti-Inflammatory Effects in Human THP-1 Monocytes and in a Mouse Model of Acute Colitis
    Article Snippet: Non-specific control siRNA (100 pmol/transfection, Life Technologies Ltd) was used as negative control.

    Article Title: Functional diversity of miR-146a-5p and TRAF6 in normal and oral cancer cells.
    Article Snippet: A non-specific control siRNA (Invitrogen, Carlsbad, CA, USA) and siRNAs against human TRAF6 (40 nM; Sigma-Aldrich, St. Louis, MO, USA) and FADD (40 nM; Santa Cruz Biotechnology, Santa Cruz, CA, USA) were used for gene silencing.

    Article Title: Gremlin Regulates Tubular Epithelial to Mesenchymal Transition via VEGFR2: Potential Role in Renal Fibrosis
    Article Snippet: Gene silencing in cultured cells was performed using either a predesigned siRNA corresponding to the human KDR/VEGFR2 cDNA sequence (s7822; Ambion) or a non-specific control siRNA (Ambion).

    Article Title: TLR3 and TLR7 RNA Sensor Activation during SARS-CoV-2 Infection
    Article Snippet: Further, siRNAs specific to human TLR3 (assay ID 107054; Thermo Fisher, Milan, Italy) and human TLR7 (assay ID 108895; Thermo Fisher, Milan, Italy), and the non-specific control siRNA (Ambion Silencer Negative Control) (Thermo Fisher, Milan, Italy), were transfected to Calu-3/MRC5 cells cultured on a 6-well plate (6 × 10 5 /well) using the Lipofectamine RNAiMAX reagent (Thermo Fisher, Milan, Italy) according to the manufacturer’s instructions.

    Article Title: Highly expressed lncRNA H19 in endometriosis promotes aerobic glycolysis and histone lactylation
    Article Snippet: Transient H19 knockdown was performed by transfecting 10 nmol/L siRNA targeting human H19 (Life Technologies, Carlsbad, CA, USA) or non-specific control siRNA (Life Technologies) into HESCs for 48 h using Lipofectamine 3000 (L3000001, Invitrogen, USA) according to the manufacturer's instructions.

    Labeling:

    Article Title: Effect of isolated keratin 3 knockdown on gene expression of primary limbal epithelial cells without and with inflammatory stimuli.
    Article Snippet: Control LECs were transfected with 150 pmol of non-specific control siRNA (Catalogue no. #4390843, Thermo Fisher Scientific, SilencerTM Select Negative Control, Waltham, USA).

    Article Title: CCN2 Binds to Tubular Epithelial Cells in the Kidney
    Article Snippet: HK2 cells were silenced using either a predesigned siRNA targeting the human EGFR or a non-specific control siRNA, both FAM-labeled (Ambion, Fisher Scientific, Waltham, MA, USA).

    Article Title: Calcium Channels as Novel Therapeutic Targets for Ovarian Cancer Stem Cells
    Article Snippet: After seeding the cells, they were transfected with siGENOME SMARTpool siRNA against CACNA1D , CACNA1F , CACNA1H , and non-specific control siRNA (each 100 nM, Thermo Fisher Scientific, USA) using Lipofectamin RNAiMax transfection reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions.

    Article Title: Activation of Protein Tyrosine Phosphatase Non-Receptor Type 2 by Spermidine Exerts Anti-Inflammatory Effects in Human THP-1 Monocytes and in a Mouse Model of Acute Colitis
    Article Snippet: Non-specific control siRNA (100 pmol/transfection, Life Technologies Ltd) was used as negative control.

    Article Title: Functional diversity of miR-146a-5p and TRAF6 in normal and oral cancer cells.
    Article Snippet: A non-specific control siRNA (Invitrogen, Carlsbad, CA, USA) and siRNAs against human TRAF6 (40 nM; Sigma-Aldrich, St. Louis, MO, USA) and FADD (40 nM; Santa Cruz Biotechnology, Santa Cruz, CA, USA) were used for gene silencing.

    Article Title: Gremlin Regulates Tubular Epithelial to Mesenchymal Transition via VEGFR2: Potential Role in Renal Fibrosis
    Article Snippet: Gene silencing in cultured cells was performed using either a predesigned siRNA corresponding to the human KDR/VEGFR2 cDNA sequence (s7822; Ambion) or a non-specific control siRNA (Ambion).

    Article Title: TLR3 and TLR7 RNA Sensor Activation during SARS-CoV-2 Infection
    Article Snippet: Further, siRNAs specific to human TLR3 (assay ID 107054; Thermo Fisher, Milan, Italy) and human TLR7 (assay ID 108895; Thermo Fisher, Milan, Italy), and the non-specific control siRNA (Ambion Silencer Negative Control) (Thermo Fisher, Milan, Italy), were transfected to Calu-3/MRC5 cells cultured on a 6-well plate (6 × 10 5 /well) using the Lipofectamine RNAiMAX reagent (Thermo Fisher, Milan, Italy) according to the manufacturer’s instructions.

    Article Title: Highly expressed lncRNA H19 in endometriosis promotes aerobic glycolysis and histone lactylation
    Article Snippet: Transient H19 knockdown was performed by transfecting 10 nmol/L siRNA targeting human H19 (Life Technologies, Carlsbad, CA, USA) or non-specific control siRNA (Life Technologies) into HESCs for 48 h using Lipofectamine 3000 (L3000001, Invitrogen, USA) according to the manufacturer's instructions.

    Expressing:

    Article Title: Effect of isolated keratin 3 knockdown on gene expression of primary limbal epithelial cells without and with inflammatory stimuli.
    Article Snippet: Control LECs were transfected with 150 pmol of non-specific control siRNA (Catalogue no. #4390843, Thermo Fisher Scientific, SilencerTM Select Negative Control, Waltham, USA).

    Article Title: CCN2 Binds to Tubular Epithelial Cells in the Kidney
    Article Snippet: HK2 cells were silenced using either a predesigned siRNA targeting the human EGFR or a non-specific control siRNA, both FAM-labeled (Ambion, Fisher Scientific, Waltham, MA, USA).

    Article Title: Calcium Channels as Novel Therapeutic Targets for Ovarian Cancer Stem Cells
    Article Snippet: After seeding the cells, they were transfected with siGENOME SMARTpool siRNA against CACNA1D , CACNA1F , CACNA1H , and non-specific control siRNA (each 100 nM, Thermo Fisher Scientific, USA) using Lipofectamin RNAiMax transfection reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions.

    Article Title: Activation of Protein Tyrosine Phosphatase Non-Receptor Type 2 by Spermidine Exerts Anti-Inflammatory Effects in Human THP-1 Monocytes and in a Mouse Model of Acute Colitis
    Article Snippet: Non-specific control siRNA (100 pmol/transfection, Life Technologies Ltd) was used as negative control.

    Article Title: Functional diversity of miR-146a-5p and TRAF6 in normal and oral cancer cells.
    Article Snippet: A non-specific control siRNA (Invitrogen, Carlsbad, CA, USA) and siRNAs against human TRAF6 (40 nM; Sigma-Aldrich, St. Louis, MO, USA) and FADD (40 nM; Santa Cruz Biotechnology, Santa Cruz, CA, USA) were used for gene silencing.

    Article Title: Gremlin Regulates Tubular Epithelial to Mesenchymal Transition via VEGFR2: Potential Role in Renal Fibrosis
    Article Snippet: Gene silencing in cultured cells was performed using either a predesigned siRNA corresponding to the human KDR/VEGFR2 cDNA sequence (s7822; Ambion) or a non-specific control siRNA (Ambion).

    Article Title: TLR3 and TLR7 RNA Sensor Activation during SARS-CoV-2 Infection
    Article Snippet: Further, siRNAs specific to human TLR3 (assay ID 107054; Thermo Fisher, Milan, Italy) and human TLR7 (assay ID 108895; Thermo Fisher, Milan, Italy), and the non-specific control siRNA (Ambion Silencer Negative Control) (Thermo Fisher, Milan, Italy), were transfected to Calu-3/MRC5 cells cultured on a 6-well plate (6 × 10 5 /well) using the Lipofectamine RNAiMAX reagent (Thermo Fisher, Milan, Italy) according to the manufacturer’s instructions.

    Article Title: Highly expressed lncRNA H19 in endometriosis promotes aerobic glycolysis and histone lactylation
    Article Snippet: Transient H19 knockdown was performed by transfecting 10 nmol/L siRNA targeting human H19 (Life Technologies, Carlsbad, CA, USA) or non-specific control siRNA (Life Technologies) into HESCs for 48 h using Lipofectamine 3000 (L3000001, Invitrogen, USA) according to the manufacturer's instructions.

    Cell Culture:

    Article Title: Effect of isolated keratin 3 knockdown on gene expression of primary limbal epithelial cells without and with inflammatory stimuli.
    Article Snippet: Control LECs were transfected with 150 pmol of non-specific control siRNA (Catalogue no. #4390843, Thermo Fisher Scientific, SilencerTM Select Negative Control, Waltham, USA).

    Article Title: CCN2 Binds to Tubular Epithelial Cells in the Kidney
    Article Snippet: HK2 cells were silenced using either a predesigned siRNA targeting the human EGFR or a non-specific control siRNA, both FAM-labeled (Ambion, Fisher Scientific, Waltham, MA, USA).

    Article Title: Calcium Channels as Novel Therapeutic Targets for Ovarian Cancer Stem Cells
    Article Snippet: After seeding the cells, they were transfected with siGENOME SMARTpool siRNA against CACNA1D , CACNA1F , CACNA1H , and non-specific control siRNA (each 100 nM, Thermo Fisher Scientific, USA) using Lipofectamin RNAiMax transfection reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions.

    Article Title: Activation of Protein Tyrosine Phosphatase Non-Receptor Type 2 by Spermidine Exerts Anti-Inflammatory Effects in Human THP-1 Monocytes and in a Mouse Model of Acute Colitis
    Article Snippet: Non-specific control siRNA (100 pmol/transfection, Life Technologies Ltd) was used as negative control.

    Article Title: Functional diversity of miR-146a-5p and TRAF6 in normal and oral cancer cells.
    Article Snippet: A non-specific control siRNA (Invitrogen, Carlsbad, CA, USA) and siRNAs against human TRAF6 (40 nM; Sigma-Aldrich, St. Louis, MO, USA) and FADD (40 nM; Santa Cruz Biotechnology, Santa Cruz, CA, USA) were used for gene silencing.

    Article Title: Gremlin Regulates Tubular Epithelial to Mesenchymal Transition via VEGFR2: Potential Role in Renal Fibrosis
    Article Snippet: Gene silencing in cultured cells was performed using either a predesigned siRNA corresponding to the human KDR/VEGFR2 cDNA sequence (s7822; Ambion) or a non-specific control siRNA (Ambion).

    Article Title: TLR3 and TLR7 RNA Sensor Activation during SARS-CoV-2 Infection
    Article Snippet: Further, siRNAs specific to human TLR3 (assay ID 107054; Thermo Fisher, Milan, Italy) and human TLR7 (assay ID 108895; Thermo Fisher, Milan, Italy), and the non-specific control siRNA (Ambion Silencer Negative Control) (Thermo Fisher, Milan, Italy), were transfected to Calu-3/MRC5 cells cultured on a 6-well plate (6 × 10 5 /well) using the Lipofectamine RNAiMAX reagent (Thermo Fisher, Milan, Italy) according to the manufacturer’s instructions.

    Article Title: Highly expressed lncRNA H19 in endometriosis promotes aerobic glycolysis and histone lactylation
    Article Snippet: Transient H19 knockdown was performed by transfecting 10 nmol/L siRNA targeting human H19 (Life Technologies, Carlsbad, CA, USA) or non-specific control siRNA (Life Technologies) into HESCs for 48 h using Lipofectamine 3000 (L3000001, Invitrogen, USA) according to the manufacturer's instructions.

    Patch Clamp:

    Article Title: Effect of isolated keratin 3 knockdown on gene expression of primary limbal epithelial cells without and with inflammatory stimuli.
    Article Snippet: Control LECs were transfected with 150 pmol of non-specific control siRNA (Catalogue no. #4390843, Thermo Fisher Scientific, SilencerTM Select Negative Control, Waltham, USA).

    Article Title: CCN2 Binds to Tubular Epithelial Cells in the Kidney
    Article Snippet: HK2 cells were silenced using either a predesigned siRNA targeting the human EGFR or a non-specific control siRNA, both FAM-labeled (Ambion, Fisher Scientific, Waltham, MA, USA).

    Article Title: Calcium Channels as Novel Therapeutic Targets for Ovarian Cancer Stem Cells
    Article Snippet: After seeding the cells, they were transfected with siGENOME SMARTpool siRNA against CACNA1D , CACNA1F , CACNA1H , and non-specific control siRNA (each 100 nM, Thermo Fisher Scientific, USA) using Lipofectamin RNAiMax transfection reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions.

    Article Title: Activation of Protein Tyrosine Phosphatase Non-Receptor Type 2 by Spermidine Exerts Anti-Inflammatory Effects in Human THP-1 Monocytes and in a Mouse Model of Acute Colitis
    Article Snippet: Non-specific control siRNA (100 pmol/transfection, Life Technologies Ltd) was used as negative control.

    Article Title: Functional diversity of miR-146a-5p and TRAF6 in normal and oral cancer cells.
    Article Snippet: A non-specific control siRNA (Invitrogen, Carlsbad, CA, USA) and siRNAs against human TRAF6 (40 nM; Sigma-Aldrich, St. Louis, MO, USA) and FADD (40 nM; Santa Cruz Biotechnology, Santa Cruz, CA, USA) were used for gene silencing.

    Article Title: Gremlin Regulates Tubular Epithelial to Mesenchymal Transition via VEGFR2: Potential Role in Renal Fibrosis
    Article Snippet: Gene silencing in cultured cells was performed using either a predesigned siRNA corresponding to the human KDR/VEGFR2 cDNA sequence (s7822; Ambion) or a non-specific control siRNA (Ambion).

    Article Title: TLR3 and TLR7 RNA Sensor Activation during SARS-CoV-2 Infection
    Article Snippet: Further, siRNAs specific to human TLR3 (assay ID 107054; Thermo Fisher, Milan, Italy) and human TLR7 (assay ID 108895; Thermo Fisher, Milan, Italy), and the non-specific control siRNA (Ambion Silencer Negative Control) (Thermo Fisher, Milan, Italy), were transfected to Calu-3/MRC5 cells cultured on a 6-well plate (6 × 10 5 /well) using the Lipofectamine RNAiMAX reagent (Thermo Fisher, Milan, Italy) according to the manufacturer’s instructions.

    Article Title: Highly expressed lncRNA H19 in endometriosis promotes aerobic glycolysis and histone lactylation
    Article Snippet: Transient H19 knockdown was performed by transfecting 10 nmol/L siRNA targeting human H19 (Life Technologies, Carlsbad, CA, USA) or non-specific control siRNA (Life Technologies) into HESCs for 48 h using Lipofectamine 3000 (L3000001, Invitrogen, USA) according to the manufacturer's instructions.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Effect of isolated keratin 3 knockdown on gene expression of primary limbal epithelial cells without and with inflammatory stimuli.
    Article Snippet: Control LECs were transfected with 150 pmol of non-specific control siRNA (Catalogue no. #4390843, Thermo Fisher Scientific, SilencerTM Select Negative Control, Waltham, USA).

    Article Title: CCN2 Binds to Tubular Epithelial Cells in the Kidney
    Article Snippet: HK2 cells were silenced using either a predesigned siRNA targeting the human EGFR or a non-specific control siRNA, both FAM-labeled (Ambion, Fisher Scientific, Waltham, MA, USA).

    Article Title: Calcium Channels as Novel Therapeutic Targets for Ovarian Cancer Stem Cells
    Article Snippet: After seeding the cells, they were transfected with siGENOME SMARTpool siRNA against CACNA1D , CACNA1F , CACNA1H , and non-specific control siRNA (each 100 nM, Thermo Fisher Scientific, USA) using Lipofectamin RNAiMax transfection reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions.

    Article Title: Activation of Protein Tyrosine Phosphatase Non-Receptor Type 2 by Spermidine Exerts Anti-Inflammatory Effects in Human THP-1 Monocytes and in a Mouse Model of Acute Colitis
    Article Snippet: Non-specific control siRNA (100 pmol/transfection, Life Technologies Ltd) was used as negative control.

    Article Title: Functional diversity of miR-146a-5p and TRAF6 in normal and oral cancer cells.
    Article Snippet: A non-specific control siRNA (Invitrogen, Carlsbad, CA, USA) and siRNAs against human TRAF6 (40 nM; Sigma-Aldrich, St. Louis, MO, USA) and FADD (40 nM; Santa Cruz Biotechnology, Santa Cruz, CA, USA) were used for gene silencing.

    Article Title: Gremlin Regulates Tubular Epithelial to Mesenchymal Transition via VEGFR2: Potential Role in Renal Fibrosis
    Article Snippet: Gene silencing in cultured cells was performed using either a predesigned siRNA corresponding to the human KDR/VEGFR2 cDNA sequence (s7822; Ambion) or a non-specific control siRNA (Ambion).

    Article Title: TLR3 and TLR7 RNA Sensor Activation during SARS-CoV-2 Infection
    Article Snippet: Further, siRNAs specific to human TLR3 (assay ID 107054; Thermo Fisher, Milan, Italy) and human TLR7 (assay ID 108895; Thermo Fisher, Milan, Italy), and the non-specific control siRNA (Ambion Silencer Negative Control) (Thermo Fisher, Milan, Italy), were transfected to Calu-3/MRC5 cells cultured on a 6-well plate (6 × 10 5 /well) using the Lipofectamine RNAiMAX reagent (Thermo Fisher, Milan, Italy) according to the manufacturer’s instructions.

    Article Title: Highly expressed lncRNA H19 in endometriosis promotes aerobic glycolysis and histone lactylation
    Article Snippet: Transient H19 knockdown was performed by transfecting 10 nmol/L siRNA targeting human H19 (Life Technologies, Carlsbad, CA, USA) or non-specific control siRNA (Life Technologies) into HESCs for 48 h using Lipofectamine 3000 (L3000001, Invitrogen, USA) according to the manufacturer's instructions.

    Quantitative RT-PCR:

    Article Title: Effect of isolated keratin 3 knockdown on gene expression of primary limbal epithelial cells without and with inflammatory stimuli.
    Article Snippet: Control LECs were transfected with 150 pmol of non-specific control siRNA (Catalogue no. #4390843, Thermo Fisher Scientific, SilencerTM Select Negative Control, Waltham, USA).

    Article Title: CCN2 Binds to Tubular Epithelial Cells in the Kidney
    Article Snippet: HK2 cells were silenced using either a predesigned siRNA targeting the human EGFR or a non-specific control siRNA, both FAM-labeled (Ambion, Fisher Scientific, Waltham, MA, USA).

    Article Title: Calcium Channels as Novel Therapeutic Targets for Ovarian Cancer Stem Cells
    Article Snippet: After seeding the cells, they were transfected with siGENOME SMARTpool siRNA against CACNA1D , CACNA1F , CACNA1H , and non-specific control siRNA (each 100 nM, Thermo Fisher Scientific, USA) using Lipofectamin RNAiMax transfection reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions.

    Article Title: Activation of Protein Tyrosine Phosphatase Non-Receptor Type 2 by Spermidine Exerts Anti-Inflammatory Effects in Human THP-1 Monocytes and in a Mouse Model of Acute Colitis
    Article Snippet: Non-specific control siRNA (100 pmol/transfection, Life Technologies Ltd) was used as negative control.

    Article Title: Functional diversity of miR-146a-5p and TRAF6 in normal and oral cancer cells.
    Article Snippet: A non-specific control siRNA (Invitrogen, Carlsbad, CA, USA) and siRNAs against human TRAF6 (40 nM; Sigma-Aldrich, St. Louis, MO, USA) and FADD (40 nM; Santa Cruz Biotechnology, Santa Cruz, CA, USA) were used for gene silencing.

    Article Title: Gremlin Regulates Tubular Epithelial to Mesenchymal Transition via VEGFR2: Potential Role in Renal Fibrosis
    Article Snippet: Gene silencing in cultured cells was performed using either a predesigned siRNA corresponding to the human KDR/VEGFR2 cDNA sequence (s7822; Ambion) or a non-specific control siRNA (Ambion).

    Article Title: TLR3 and TLR7 RNA Sensor Activation during SARS-CoV-2 Infection
    Article Snippet: Further, siRNAs specific to human TLR3 (assay ID 107054; Thermo Fisher, Milan, Italy) and human TLR7 (assay ID 108895; Thermo Fisher, Milan, Italy), and the non-specific control siRNA (Ambion Silencer Negative Control) (Thermo Fisher, Milan, Italy), were transfected to Calu-3/MRC5 cells cultured on a 6-well plate (6 × 10 5 /well) using the Lipofectamine RNAiMAX reagent (Thermo Fisher, Milan, Italy) according to the manufacturer’s instructions.

    Article Title: Highly expressed lncRNA H19 in endometriosis promotes aerobic glycolysis and histone lactylation
    Article Snippet: Transient H19 knockdown was performed by transfecting 10 nmol/L siRNA targeting human H19 (Life Technologies, Carlsbad, CA, USA) or non-specific control siRNA (Life Technologies) into HESCs for 48 h using Lipofectamine 3000 (L3000001, Invitrogen, USA) according to the manufacturer's instructions.

    Western Blot:

    Article Title: Effect of isolated keratin 3 knockdown on gene expression of primary limbal epithelial cells without and with inflammatory stimuli.
    Article Snippet: Control LECs were transfected with 150 pmol of non-specific control siRNA (Catalogue no. #4390843, Thermo Fisher Scientific, SilencerTM Select Negative Control, Waltham, USA).

    Article Title: CCN2 Binds to Tubular Epithelial Cells in the Kidney
    Article Snippet: HK2 cells were silenced using either a predesigned siRNA targeting the human EGFR or a non-specific control siRNA, both FAM-labeled (Ambion, Fisher Scientific, Waltham, MA, USA).

    Article Title: Calcium Channels as Novel Therapeutic Targets for Ovarian Cancer Stem Cells
    Article Snippet: After seeding the cells, they were transfected with siGENOME SMARTpool siRNA against CACNA1D , CACNA1F , CACNA1H , and non-specific control siRNA (each 100 nM, Thermo Fisher Scientific, USA) using Lipofectamin RNAiMax transfection reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions.

    Article Title: Activation of Protein Tyrosine Phosphatase Non-Receptor Type 2 by Spermidine Exerts Anti-Inflammatory Effects in Human THP-1 Monocytes and in a Mouse Model of Acute Colitis
    Article Snippet: Non-specific control siRNA (100 pmol/transfection, Life Technologies Ltd) was used as negative control.

    Article Title: Functional diversity of miR-146a-5p and TRAF6 in normal and oral cancer cells.
    Article Snippet: A non-specific control siRNA (Invitrogen, Carlsbad, CA, USA) and siRNAs against human TRAF6 (40 nM; Sigma-Aldrich, St. Louis, MO, USA) and FADD (40 nM; Santa Cruz Biotechnology, Santa Cruz, CA, USA) were used for gene silencing.

    Article Title: Gremlin Regulates Tubular Epithelial to Mesenchymal Transition via VEGFR2: Potential Role in Renal Fibrosis
    Article Snippet: Gene silencing in cultured cells was performed using either a predesigned siRNA corresponding to the human KDR/VEGFR2 cDNA sequence (s7822; Ambion) or a non-specific control siRNA (Ambion).

    Article Title: TLR3 and TLR7 RNA Sensor Activation during SARS-CoV-2 Infection
    Article Snippet: Further, siRNAs specific to human TLR3 (assay ID 107054; Thermo Fisher, Milan, Italy) and human TLR7 (assay ID 108895; Thermo Fisher, Milan, Italy), and the non-specific control siRNA (Ambion Silencer Negative Control) (Thermo Fisher, Milan, Italy), were transfected to Calu-3/MRC5 cells cultured on a 6-well plate (6 × 10 5 /well) using the Lipofectamine RNAiMAX reagent (Thermo Fisher, Milan, Italy) according to the manufacturer’s instructions.

    Article Title: Highly expressed lncRNA H19 in endometriosis promotes aerobic glycolysis and histone lactylation
    Article Snippet: Transient H19 knockdown was performed by transfecting 10 nmol/L siRNA targeting human H19 (Life Technologies, Carlsbad, CA, USA) or non-specific control siRNA (Life Technologies) into HESCs for 48 h using Lipofectamine 3000 (L3000001, Invitrogen, USA) according to the manufacturer's instructions.

    Sequencing:

    Article Title: Effect of isolated keratin 3 knockdown on gene expression of primary limbal epithelial cells without and with inflammatory stimuli.
    Article Snippet: Control LECs were transfected with 150 pmol of non-specific control siRNA (Catalogue no. #4390843, Thermo Fisher Scientific, SilencerTM Select Negative Control, Waltham, USA).

    Article Title: CCN2 Binds to Tubular Epithelial Cells in the Kidney
    Article Snippet: HK2 cells were silenced using either a predesigned siRNA targeting the human EGFR or a non-specific control siRNA, both FAM-labeled (Ambion, Fisher Scientific, Waltham, MA, USA).

    Article Title: Calcium Channels as Novel Therapeutic Targets for Ovarian Cancer Stem Cells
    Article Snippet: After seeding the cells, they were transfected with siGENOME SMARTpool siRNA against CACNA1D , CACNA1F , CACNA1H , and non-specific control siRNA (each 100 nM, Thermo Fisher Scientific, USA) using Lipofectamin RNAiMax transfection reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions.

    Article Title: Activation of Protein Tyrosine Phosphatase Non-Receptor Type 2 by Spermidine Exerts Anti-Inflammatory Effects in Human THP-1 Monocytes and in a Mouse Model of Acute Colitis
    Article Snippet: Non-specific control siRNA (100 pmol/transfection, Life Technologies Ltd) was used as negative control.

    Article Title: Functional diversity of miR-146a-5p and TRAF6 in normal and oral cancer cells.
    Article Snippet: A non-specific control siRNA (Invitrogen, Carlsbad, CA, USA) and siRNAs against human TRAF6 (40 nM; Sigma-Aldrich, St. Louis, MO, USA) and FADD (40 nM; Santa Cruz Biotechnology, Santa Cruz, CA, USA) were used for gene silencing.

    Article Title: Gremlin Regulates Tubular Epithelial to Mesenchymal Transition via VEGFR2: Potential Role in Renal Fibrosis
    Article Snippet: Gene silencing in cultured cells was performed using either a predesigned siRNA corresponding to the human KDR/VEGFR2 cDNA sequence (s7822; Ambion) or a non-specific control siRNA (Ambion).

    Article Title: TLR3 and TLR7 RNA Sensor Activation during SARS-CoV-2 Infection
    Article Snippet: Further, siRNAs specific to human TLR3 (assay ID 107054; Thermo Fisher, Milan, Italy) and human TLR7 (assay ID 108895; Thermo Fisher, Milan, Italy), and the non-specific control siRNA (Ambion Silencer Negative Control) (Thermo Fisher, Milan, Italy), were transfected to Calu-3/MRC5 cells cultured on a 6-well plate (6 × 10 5 /well) using the Lipofectamine RNAiMAX reagent (Thermo Fisher, Milan, Italy) according to the manufacturer’s instructions.

    Article Title: Highly expressed lncRNA H19 in endometriosis promotes aerobic glycolysis and histone lactylation
    Article Snippet: Transient H19 knockdown was performed by transfecting 10 nmol/L siRNA targeting human H19 (Life Technologies, Carlsbad, CA, USA) or non-specific control siRNA (Life Technologies) into HESCs for 48 h using Lipofectamine 3000 (L3000001, Invitrogen, USA) according to the manufacturer's instructions.

    Isolation:

    Article Title: Effect of isolated keratin 3 knockdown on gene expression of primary limbal epithelial cells without and with inflammatory stimuli.
    Article Snippet: Control LECs were transfected with 150 pmol of non-specific control siRNA (Catalogue no. #4390843, Thermo Fisher Scientific, SilencerTM Select Negative Control, Waltham, USA).

    Article Title: CCN2 Binds to Tubular Epithelial Cells in the Kidney
    Article Snippet: HK2 cells were silenced using either a predesigned siRNA targeting the human EGFR or a non-specific control siRNA, both FAM-labeled (Ambion, Fisher Scientific, Waltham, MA, USA).

    Article Title: Calcium Channels as Novel Therapeutic Targets for Ovarian Cancer Stem Cells
    Article Snippet: After seeding the cells, they were transfected with siGENOME SMARTpool siRNA against CACNA1D , CACNA1F , CACNA1H , and non-specific control siRNA (each 100 nM, Thermo Fisher Scientific, USA) using Lipofectamin RNAiMax transfection reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions.

    Article Title: Activation of Protein Tyrosine Phosphatase Non-Receptor Type 2 by Spermidine Exerts Anti-Inflammatory Effects in Human THP-1 Monocytes and in a Mouse Model of Acute Colitis
    Article Snippet: Non-specific control siRNA (100 pmol/transfection, Life Technologies Ltd) was used as negative control.

    Article Title: Functional diversity of miR-146a-5p and TRAF6 in normal and oral cancer cells.
    Article Snippet: A non-specific control siRNA (Invitrogen, Carlsbad, CA, USA) and siRNAs against human TRAF6 (40 nM; Sigma-Aldrich, St. Louis, MO, USA) and FADD (40 nM; Santa Cruz Biotechnology, Santa Cruz, CA, USA) were used for gene silencing.

    Article Title: Gremlin Regulates Tubular Epithelial to Mesenchymal Transition via VEGFR2: Potential Role in Renal Fibrosis
    Article Snippet: Gene silencing in cultured cells was performed using either a predesigned siRNA corresponding to the human KDR/VEGFR2 cDNA sequence (s7822; Ambion) or a non-specific control siRNA (Ambion).

    Article Title: TLR3 and TLR7 RNA Sensor Activation during SARS-CoV-2 Infection
    Article Snippet: Further, siRNAs specific to human TLR3 (assay ID 107054; Thermo Fisher, Milan, Italy) and human TLR7 (assay ID 108895; Thermo Fisher, Milan, Italy), and the non-specific control siRNA (Ambion Silencer Negative Control) (Thermo Fisher, Milan, Italy), were transfected to Calu-3/MRC5 cells cultured on a 6-well plate (6 × 10 5 /well) using the Lipofectamine RNAiMAX reagent (Thermo Fisher, Milan, Italy) according to the manufacturer’s instructions.

    Article Title: Highly expressed lncRNA H19 in endometriosis promotes aerobic glycolysis and histone lactylation
    Article Snippet: Transient H19 knockdown was performed by transfecting 10 nmol/L siRNA targeting human H19 (Life Technologies, Carlsbad, CA, USA) or non-specific control siRNA (Life Technologies) into HESCs for 48 h using Lipofectamine 3000 (L3000001, Invitrogen, USA) according to the manufacturer's instructions.

    Real-time Polymerase Chain Reaction:

    Article Title: Effect of isolated keratin 3 knockdown on gene expression of primary limbal epithelial cells without and with inflammatory stimuli.
    Article Snippet: Control LECs were transfected with 150 pmol of non-specific control siRNA (Catalogue no. #4390843, Thermo Fisher Scientific, SilencerTM Select Negative Control, Waltham, USA).

    Article Title: CCN2 Binds to Tubular Epithelial Cells in the Kidney
    Article Snippet: HK2 cells were silenced using either a predesigned siRNA targeting the human EGFR or a non-specific control siRNA, both FAM-labeled (Ambion, Fisher Scientific, Waltham, MA, USA).

    Article Title: Calcium Channels as Novel Therapeutic Targets for Ovarian Cancer Stem Cells
    Article Snippet: After seeding the cells, they were transfected with siGENOME SMARTpool siRNA against CACNA1D , CACNA1F , CACNA1H , and non-specific control siRNA (each 100 nM, Thermo Fisher Scientific, USA) using Lipofectamin RNAiMax transfection reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions.

    Article Title: Activation of Protein Tyrosine Phosphatase Non-Receptor Type 2 by Spermidine Exerts Anti-Inflammatory Effects in Human THP-1 Monocytes and in a Mouse Model of Acute Colitis
    Article Snippet: Non-specific control siRNA (100 pmol/transfection, Life Technologies Ltd) was used as negative control.

    Article Title: Functional diversity of miR-146a-5p and TRAF6 in normal and oral cancer cells.
    Article Snippet: A non-specific control siRNA (Invitrogen, Carlsbad, CA, USA) and siRNAs against human TRAF6 (40 nM; Sigma-Aldrich, St. Louis, MO, USA) and FADD (40 nM; Santa Cruz Biotechnology, Santa Cruz, CA, USA) were used for gene silencing.

    Article Title: Gremlin Regulates Tubular Epithelial to Mesenchymal Transition via VEGFR2: Potential Role in Renal Fibrosis
    Article Snippet: Gene silencing in cultured cells was performed using either a predesigned siRNA corresponding to the human KDR/VEGFR2 cDNA sequence (s7822; Ambion) or a non-specific control siRNA (Ambion).

    Article Title: TLR3 and TLR7 RNA Sensor Activation during SARS-CoV-2 Infection
    Article Snippet: Further, siRNAs specific to human TLR3 (assay ID 107054; Thermo Fisher, Milan, Italy) and human TLR7 (assay ID 108895; Thermo Fisher, Milan, Italy), and the non-specific control siRNA (Ambion Silencer Negative Control) (Thermo Fisher, Milan, Italy), were transfected to Calu-3/MRC5 cells cultured on a 6-well plate (6 × 10 5 /well) using the Lipofectamine RNAiMAX reagent (Thermo Fisher, Milan, Italy) according to the manufacturer’s instructions.

    Article Title: Highly expressed lncRNA H19 in endometriosis promotes aerobic glycolysis and histone lactylation
    Article Snippet: Transient H19 knockdown was performed by transfecting 10 nmol/L siRNA targeting human H19 (Life Technologies, Carlsbad, CA, USA) or non-specific control siRNA (Life Technologies) into HESCs for 48 h using Lipofectamine 3000 (L3000001, Invitrogen, USA) according to the manufacturer's instructions.

    Incubation:

    Article Title: Effect of isolated keratin 3 knockdown on gene expression of primary limbal epithelial cells without and with inflammatory stimuli.
    Article Snippet: Control LECs were transfected with 150 pmol of non-specific control siRNA (Catalogue no. #4390843, Thermo Fisher Scientific, SilencerTM Select Negative Control, Waltham, USA).

    Article Title: CCN2 Binds to Tubular Epithelial Cells in the Kidney
    Article Snippet: HK2 cells were silenced using either a predesigned siRNA targeting the human EGFR or a non-specific control siRNA, both FAM-labeled (Ambion, Fisher Scientific, Waltham, MA, USA).

    Article Title: Calcium Channels as Novel Therapeutic Targets for Ovarian Cancer Stem Cells
    Article Snippet: After seeding the cells, they were transfected with siGENOME SMARTpool siRNA against CACNA1D , CACNA1F , CACNA1H , and non-specific control siRNA (each 100 nM, Thermo Fisher Scientific, USA) using Lipofectamin RNAiMax transfection reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions.

    Article Title: Activation of Protein Tyrosine Phosphatase Non-Receptor Type 2 by Spermidine Exerts Anti-Inflammatory Effects in Human THP-1 Monocytes and in a Mouse Model of Acute Colitis
    Article Snippet: Non-specific control siRNA (100 pmol/transfection, Life Technologies Ltd) was used as negative control.

    Article Title: Functional diversity of miR-146a-5p and TRAF6 in normal and oral cancer cells.
    Article Snippet: A non-specific control siRNA (Invitrogen, Carlsbad, CA, USA) and siRNAs against human TRAF6 (40 nM; Sigma-Aldrich, St. Louis, MO, USA) and FADD (40 nM; Santa Cruz Biotechnology, Santa Cruz, CA, USA) were used for gene silencing.

    Article Title: Gremlin Regulates Tubular Epithelial to Mesenchymal Transition via VEGFR2: Potential Role in Renal Fibrosis
    Article Snippet: Gene silencing in cultured cells was performed using either a predesigned siRNA corresponding to the human KDR/VEGFR2 cDNA sequence (s7822; Ambion) or a non-specific control siRNA (Ambion).

    Article Title: TLR3 and TLR7 RNA Sensor Activation during SARS-CoV-2 Infection
    Article Snippet: Further, siRNAs specific to human TLR3 (assay ID 107054; Thermo Fisher, Milan, Italy) and human TLR7 (assay ID 108895; Thermo Fisher, Milan, Italy), and the non-specific control siRNA (Ambion Silencer Negative Control) (Thermo Fisher, Milan, Italy), were transfected to Calu-3/MRC5 cells cultured on a 6-well plate (6 × 10 5 /well) using the Lipofectamine RNAiMAX reagent (Thermo Fisher, Milan, Italy) according to the manufacturer’s instructions.

    Article Title: Highly expressed lncRNA H19 in endometriosis promotes aerobic glycolysis and histone lactylation
    Article Snippet: Transient H19 knockdown was performed by transfecting 10 nmol/L siRNA targeting human H19 (Life Technologies, Carlsbad, CA, USA) or non-specific control siRNA (Life Technologies) into HESCs for 48 h using Lipofectamine 3000 (L3000001, Invitrogen, USA) according to the manufacturer's instructions.

    Marker:

    Article Title: Effect of isolated keratin 3 knockdown on gene expression of primary limbal epithelial cells without and with inflammatory stimuli.
    Article Snippet: Control LECs were transfected with 150 pmol of non-specific control siRNA (Catalogue no. #4390843, Thermo Fisher Scientific, SilencerTM Select Negative Control, Waltham, USA).

    Article Title: CCN2 Binds to Tubular Epithelial Cells in the Kidney
    Article Snippet: HK2 cells were silenced using either a predesigned siRNA targeting the human EGFR or a non-specific control siRNA, both FAM-labeled (Ambion, Fisher Scientific, Waltham, MA, USA).

    Article Title: Calcium Channels as Novel Therapeutic Targets for Ovarian Cancer Stem Cells
    Article Snippet: After seeding the cells, they were transfected with siGENOME SMARTpool siRNA against CACNA1D , CACNA1F , CACNA1H , and non-specific control siRNA (each 100 nM, Thermo Fisher Scientific, USA) using Lipofectamin RNAiMax transfection reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions.

    Article Title: Activation of Protein Tyrosine Phosphatase Non-Receptor Type 2 by Spermidine Exerts Anti-Inflammatory Effects in Human THP-1 Monocytes and in a Mouse Model of Acute Colitis
    Article Snippet: Non-specific control siRNA (100 pmol/transfection, Life Technologies Ltd) was used as negative control.

    Article Title: Functional diversity of miR-146a-5p and TRAF6 in normal and oral cancer cells.
    Article Snippet: A non-specific control siRNA (Invitrogen, Carlsbad, CA, USA) and siRNAs against human TRAF6 (40 nM; Sigma-Aldrich, St. Louis, MO, USA) and FADD (40 nM; Santa Cruz Biotechnology, Santa Cruz, CA, USA) were used for gene silencing.

    Article Title: Gremlin Regulates Tubular Epithelial to Mesenchymal Transition via VEGFR2: Potential Role in Renal Fibrosis
    Article Snippet: Gene silencing in cultured cells was performed using either a predesigned siRNA corresponding to the human KDR/VEGFR2 cDNA sequence (s7822; Ambion) or a non-specific control siRNA (Ambion).

    Article Title: TLR3 and TLR7 RNA Sensor Activation during SARS-CoV-2 Infection
    Article Snippet: Further, siRNAs specific to human TLR3 (assay ID 107054; Thermo Fisher, Milan, Italy) and human TLR7 (assay ID 108895; Thermo Fisher, Milan, Italy), and the non-specific control siRNA (Ambion Silencer Negative Control) (Thermo Fisher, Milan, Italy), were transfected to Calu-3/MRC5 cells cultured on a 6-well plate (6 × 10 5 /well) using the Lipofectamine RNAiMAX reagent (Thermo Fisher, Milan, Italy) according to the manufacturer’s instructions.

    Article Title: Highly expressed lncRNA H19 in endometriosis promotes aerobic glycolysis and histone lactylation
    Article Snippet: Transient H19 knockdown was performed by transfecting 10 nmol/L siRNA targeting human H19 (Life Technologies, Carlsbad, CA, USA) or non-specific control siRNA (Life Technologies) into HESCs for 48 h using Lipofectamine 3000 (L3000001, Invitrogen, USA) according to the manufacturer's instructions.

    Confocal Microscopy:

    Article Title: Effect of isolated keratin 3 knockdown on gene expression of primary limbal epithelial cells without and with inflammatory stimuli.
    Article Snippet: Control LECs were transfected with 150 pmol of non-specific control siRNA (Catalogue no. #4390843, Thermo Fisher Scientific, SilencerTM Select Negative Control, Waltham, USA).

    Article Title: CCN2 Binds to Tubular Epithelial Cells in the Kidney
    Article Snippet: HK2 cells were silenced using either a predesigned siRNA targeting the human EGFR or a non-specific control siRNA, both FAM-labeled (Ambion, Fisher Scientific, Waltham, MA, USA).

    Article Title: Calcium Channels as Novel Therapeutic Targets for Ovarian Cancer Stem Cells
    Article Snippet: After seeding the cells, they were transfected with siGENOME SMARTpool siRNA against CACNA1D , CACNA1F , CACNA1H , and non-specific control siRNA (each 100 nM, Thermo Fisher Scientific, USA) using Lipofectamin RNAiMax transfection reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions.

    Article Title: Activation of Protein Tyrosine Phosphatase Non-Receptor Type 2 by Spermidine Exerts Anti-Inflammatory Effects in Human THP-1 Monocytes and in a Mouse Model of Acute Colitis
    Article Snippet: Non-specific control siRNA (100 pmol/transfection, Life Technologies Ltd) was used as negative control.

    Article Title: Functional diversity of miR-146a-5p and TRAF6 in normal and oral cancer cells.
    Article Snippet: A non-specific control siRNA (Invitrogen, Carlsbad, CA, USA) and siRNAs against human TRAF6 (40 nM; Sigma-Aldrich, St. Louis, MO, USA) and FADD (40 nM; Santa Cruz Biotechnology, Santa Cruz, CA, USA) were used for gene silencing.

    Article Title: Gremlin Regulates Tubular Epithelial to Mesenchymal Transition via VEGFR2: Potential Role in Renal Fibrosis
    Article Snippet: Gene silencing in cultured cells was performed using either a predesigned siRNA corresponding to the human KDR/VEGFR2 cDNA sequence (s7822; Ambion) or a non-specific control siRNA (Ambion).

    Article Title: TLR3 and TLR7 RNA Sensor Activation during SARS-CoV-2 Infection
    Article Snippet: Further, siRNAs specific to human TLR3 (assay ID 107054; Thermo Fisher, Milan, Italy) and human TLR7 (assay ID 108895; Thermo Fisher, Milan, Italy), and the non-specific control siRNA (Ambion Silencer Negative Control) (Thermo Fisher, Milan, Italy), were transfected to Calu-3/MRC5 cells cultured on a 6-well plate (6 × 10 5 /well) using the Lipofectamine RNAiMAX reagent (Thermo Fisher, Milan, Italy) according to the manufacturer’s instructions.

    Article Title: Highly expressed lncRNA H19 in endometriosis promotes aerobic glycolysis and histone lactylation
    Article Snippet: Transient H19 knockdown was performed by transfecting 10 nmol/L siRNA targeting human H19 (Life Technologies, Carlsbad, CA, USA) or non-specific control siRNA (Life Technologies) into HESCs for 48 h using Lipofectamine 3000 (L3000001, Invitrogen, USA) according to the manufacturer's instructions.



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    Suppression of M2 polarization through RARγ inhibition. ( A , B ) PMA-treated THP-1 cells were cultured in the M2 polarization-inducing medium containing IL-4 and IL-13, with or without the RARγ inhibitor LY-2955303. Cells were harvested and analyzed for M2 marker expression at the mRNA level for MRC1 , TGC , B7H1 , and IL-10 ( B ), and the protein level for CD206, TGM2, and PD-L1. ( C ) PMA-treated THP-1 cells were transfected with <t>siRNA</t> targeting RARγ and analyzed for protein expression of RARγ and M2 markers, including TGM2 and PDL-1. Statistical significance was expressed by asterisks (*, p < 0.05) compared with the untreated control, and by hashtags (#, p < 0.05) compared with the IL-4/IL-13-treated group.
    Non Specific Control Sirna, supplied by Eurofins, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/non-specific+control+sirna/non+targeting+negative+control+shrna/pm40622913-69-24-30
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    Shanghai GenePharma sirnas specifically targeting acta2 and vegfa, as well as non-targeting control
    Suppression of M2 polarization through RARγ inhibition. ( A , B ) PMA-treated THP-1 cells were cultured in the M2 polarization-inducing medium containing IL-4 and IL-13, with or without the RARγ inhibitor LY-2955303. Cells were harvested and analyzed for M2 marker expression at the mRNA level for MRC1 , TGC , B7H1 , and IL-10 ( B ), and the protein level for CD206, TGM2, and PD-L1. ( C ) PMA-treated THP-1 cells were transfected with <t>siRNA</t> targeting RARγ and analyzed for protein expression of RARγ and M2 markers, including TGM2 and PDL-1. Statistical significance was expressed by asterisks (*, p < 0.05) compared with the untreated control, and by hashtags (#, p < 0.05) compared with the IL-4/IL-13-treated group.
    Sirnas Specifically Targeting Acta2 And Vegfa, As Well As Non Targeting Control, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Ribobio co non-specific control sirna
    Effects of tRF Ala−AGC−3−M8 on ERK 1/2 -p70S6K pathway in BV-2 cells. (A, I) qRT-PCR analysis of tRF Ala−AGC−3−M8 <t>and</t> <t>EphA7</t> in BV-2 cells after transfection with tRF Ala−AGC−3−M8 -mimic or <t>EphA7-siRNA</t> (n = 6, biologically independent samples). (B-D, J-L) Western blot analysis of EphA7 and phosphorylation of ERK 1/2 and p70S6K (T421/S424) in BV-2 cells (n = 6, biologically independent samples). (E, M) Representative light microscopy images of BV-2 cells morphological features; Scale bar: 75μm (overview), 20μm (insert). (F-H, N-P) Immunofluorescence localization an mean fluorescence intensity of iNOS in BV-2 cells ( n = 6, biologically independent samples); Scale bar: 75μm (overview), 20μm (insert); Statistical significance was assessed using Student’s test (A and I) and one-way ANOVA (D, L, G and O) (P values are indicated). All data are presented as the means ± SEM. Each data point represents the average of 3 technical replicates
    Non Specific Control Sirna, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    OriGene yy1 (human) sirna duplexes and one non-specific (scrambled) negative control
    Effects of tRF Ala−AGC−3−M8 on ERK 1/2 -p70S6K pathway in BV-2 cells. (A, I) qRT-PCR analysis of tRF Ala−AGC−3−M8 <t>and</t> <t>EphA7</t> in BV-2 cells after transfection with tRF Ala−AGC−3−M8 -mimic or <t>EphA7-siRNA</t> (n = 6, biologically independent samples). (B-D, J-L) Western blot analysis of EphA7 and phosphorylation of ERK 1/2 and p70S6K (T421/S424) in BV-2 cells (n = 6, biologically independent samples). (E, M) Representative light microscopy images of BV-2 cells morphological features; Scale bar: 75μm (overview), 20μm (insert). (F-H, N-P) Immunofluorescence localization an mean fluorescence intensity of iNOS in BV-2 cells ( n = 6, biologically independent samples); Scale bar: 75μm (overview), 20μm (insert); Statistical significance was assessed using Student’s test (A and I) and one-way ANOVA (D, L, G and O) (P values are indicated). All data are presented as the means ± SEM. Each data point represents the average of 3 technical replicates
    Yy1 (Human) Sirna Duplexes And One Non Specific (Scrambled) Negative Control, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Suppression of M2 polarization through RARγ inhibition. ( A , B ) PMA-treated THP-1 cells were cultured in the M2 polarization-inducing medium containing IL-4 and IL-13, with or without the RARγ inhibitor LY-2955303. Cells were harvested and analyzed for M2 marker expression at the mRNA level for MRC1 , TGC , B7H1 , and IL-10 ( B ), and the protein level for CD206, TGM2, and PD-L1. ( C ) PMA-treated THP-1 cells were transfected with siRNA targeting RARγ and analyzed for protein expression of RARγ and M2 markers, including TGM2 and PDL-1. Statistical significance was expressed by asterisks (*, p < 0.05) compared with the untreated control, and by hashtags (#, p < 0.05) compared with the IL-4/IL-13-treated group.

    Journal: Molecules

    Article Title: Targeting RARγ Decreases Immunosuppressive Macrophage Polarization and Reduces Tumor Growth

    doi: 10.3390/molecules30153099

    Figure Lengend Snippet: Suppression of M2 polarization through RARγ inhibition. ( A , B ) PMA-treated THP-1 cells were cultured in the M2 polarization-inducing medium containing IL-4 and IL-13, with or without the RARγ inhibitor LY-2955303. Cells were harvested and analyzed for M2 marker expression at the mRNA level for MRC1 , TGC , B7H1 , and IL-10 ( B ), and the protein level for CD206, TGM2, and PD-L1. ( C ) PMA-treated THP-1 cells were transfected with siRNA targeting RARγ and analyzed for protein expression of RARγ and M2 markers, including TGM2 and PDL-1. Statistical significance was expressed by asterisks (*, p < 0.05) compared with the untreated control, and by hashtags (#, p < 0.05) compared with the IL-4/IL-13-treated group.

    Article Snippet: M0 THP-1 cells were transfected with siRNA targeting RARG or non-specific control siRNA (Bioneer, Daejeon, South Korea) using Lipofectamine RNAiMax Transfection reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions.

    Techniques: Inhibition, Cell Culture, Marker, Expressing, Transfection, Control

    Effects of tRF Ala−AGC−3−M8 on ERK 1/2 -p70S6K pathway in BV-2 cells. (A, I) qRT-PCR analysis of tRF Ala−AGC−3−M8 and EphA7 in BV-2 cells after transfection with tRF Ala−AGC−3−M8 -mimic or EphA7-siRNA (n = 6, biologically independent samples). (B-D, J-L) Western blot analysis of EphA7 and phosphorylation of ERK 1/2 and p70S6K (T421/S424) in BV-2 cells (n = 6, biologically independent samples). (E, M) Representative light microscopy images of BV-2 cells morphological features; Scale bar: 75μm (overview), 20μm (insert). (F-H, N-P) Immunofluorescence localization an mean fluorescence intensity of iNOS in BV-2 cells ( n = 6, biologically independent samples); Scale bar: 75μm (overview), 20μm (insert); Statistical significance was assessed using Student’s test (A and I) and one-way ANOVA (D, L, G and O) (P values are indicated). All data are presented as the means ± SEM. Each data point represents the average of 3 technical replicates

    Journal: Alzheimer's Research & Therapy

    Article Title: tRF Ala-AGC-3-M8 attenuates neuroinflammation and neuronal damage in Alzheimer’s disease via the EphA7-ERK 1/2 -p70S6K signaling pathway

    doi: 10.1186/s13195-025-01734-6

    Figure Lengend Snippet: Effects of tRF Ala−AGC−3−M8 on ERK 1/2 -p70S6K pathway in BV-2 cells. (A, I) qRT-PCR analysis of tRF Ala−AGC−3−M8 and EphA7 in BV-2 cells after transfection with tRF Ala−AGC−3−M8 -mimic or EphA7-siRNA (n = 6, biologically independent samples). (B-D, J-L) Western blot analysis of EphA7 and phosphorylation of ERK 1/2 and p70S6K (T421/S424) in BV-2 cells (n = 6, biologically independent samples). (E, M) Representative light microscopy images of BV-2 cells morphological features; Scale bar: 75μm (overview), 20μm (insert). (F-H, N-P) Immunofluorescence localization an mean fluorescence intensity of iNOS in BV-2 cells ( n = 6, biologically independent samples); Scale bar: 75μm (overview), 20μm (insert); Statistical significance was assessed using Student’s test (A and I) and one-way ANOVA (D, L, G and O) (P values are indicated). All data are presented as the means ± SEM. Each data point represents the average of 3 technical replicates

    Article Snippet: EphA7-targeting siRNA (EphA7-siRNA) and non-specific control siRNA (NC-siRNA), as well as chemically synthesized double-stranded tRF Ala-AGC-3-M8 mimic (tRF Ala-AGC-3-M8 -mimic) and non-specific control mimics (NC-mimic), were purchased from RIBOBIO Biotechnology Co., Ltd., China.

    Techniques: Quantitative RT-PCR, Transfection, Western Blot, Phospho-proteomics, Light Microscopy, Immunofluorescence, Fluorescence

    Effects of tRF Ala−AGC−3−M8 on ERK 1/2 -p70S6K pathway in HT22 cells. (A) qRT-PCR analysis of tRF Ala−AGC−3−M8 and EphA7 in HT22 cells after transfected with tRF Ala−AGC−3−M8 -mimic or EphA7-siRNA ( n = 6, biologically independent samples). (B-C) Western blot analysis of EphA7 expression and phosphorylation levels of ERK 1/2 and p70S6K (T421/S424) ( n = 6, biologically independent samples). (D) Representative image of HT22 morphological features; Scale bar: 75μm (overview), 20μm (insert). (E, F, G) Immunofluorescence localization and mean fluorescence intensity of p-tau (Red) in HT22 cells ( n = 6, biologically independent samples), p-tau was labelled with solid white line in zoomed-in immunofluorescence image; Scale bar: 75μm (overview), 20μm (insert); Statistical significance was assessed using Student’s test (A) and one-way ANOVA (C and F) (P values are indicated). All data are presented as the means ± SEM. Each data point represents the average of 3 technical replicates

    Journal: Alzheimer's Research & Therapy

    Article Title: tRF Ala-AGC-3-M8 attenuates neuroinflammation and neuronal damage in Alzheimer’s disease via the EphA7-ERK 1/2 -p70S6K signaling pathway

    doi: 10.1186/s13195-025-01734-6

    Figure Lengend Snippet: Effects of tRF Ala−AGC−3−M8 on ERK 1/2 -p70S6K pathway in HT22 cells. (A) qRT-PCR analysis of tRF Ala−AGC−3−M8 and EphA7 in HT22 cells after transfected with tRF Ala−AGC−3−M8 -mimic or EphA7-siRNA ( n = 6, biologically independent samples). (B-C) Western blot analysis of EphA7 expression and phosphorylation levels of ERK 1/2 and p70S6K (T421/S424) ( n = 6, biologically independent samples). (D) Representative image of HT22 morphological features; Scale bar: 75μm (overview), 20μm (insert). (E, F, G) Immunofluorescence localization and mean fluorescence intensity of p-tau (Red) in HT22 cells ( n = 6, biologically independent samples), p-tau was labelled with solid white line in zoomed-in immunofluorescence image; Scale bar: 75μm (overview), 20μm (insert); Statistical significance was assessed using Student’s test (A) and one-way ANOVA (C and F) (P values are indicated). All data are presented as the means ± SEM. Each data point represents the average of 3 technical replicates

    Article Snippet: EphA7-targeting siRNA (EphA7-siRNA) and non-specific control siRNA (NC-siRNA), as well as chemically synthesized double-stranded tRF Ala-AGC-3-M8 mimic (tRF Ala-AGC-3-M8 -mimic) and non-specific control mimics (NC-mimic), were purchased from RIBOBIO Biotechnology Co., Ltd., China.

    Techniques: Quantitative RT-PCR, Transfection, Western Blot, Expressing, Phospho-proteomics, Immunofluorescence, Fluorescence